概述

  • 产品名称人CD62E ELISA试剂盒(Selectin)
    参阅全部 CD62E 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 30 pg/ml
  • 范围
    24.69 pg/ml - 18000 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.39 83% - 103%
    Serum 93.75 83% - 102%
    Plasma 95.38 84% - 105%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD62E (Selectin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62E in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CD62E coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • 序列相似性Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CD62 antigen-like family member E
    • CD62E
    • CD62E antigen
    • E selectin
    • E-selectin
    • ELAM
    • ELAM-1
    • ELAM1
    • Endothelial leukocyte adhesion molecule 1
    • ESEL
    • LECAM2
    • Leukocyte endothelial cell adhesion molecule 2
    • Leukocyte-endothelial cell adhesion molecule 2
    • LYAM2_HUMAN
    • Sele
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD62E ELISA Kit (Selectin) 图像

  • Representative Standard Curve using ab100512.

  • Representative Standard Curve using ab100512.

实验方案

Human CD62E ELISA Kit (Selectin) (ab100512)参考文献

This product has been referenced in:
  • Chitalia N  et al. Impact of vitamin D supplementation on arterial vasomotion, stiffness and endothelial biomarkers in chronic kidney disease patients. PLoS One 9:e91363 (2014). Read more (PubMed: 24646518) »

See 1 Publication for this product

Product Wall

Yes, I can confirm that EDTA, Heparin, or Citrate collected plasma can be used with the ab100512 CD62E Human ELISA Kit

Thank you for contacting us.

All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

I hope this information has been of help. If your require any further information please do not...

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Thank you for your inquiry and I apologize for the delay while I was awaiting more information from our testing laboratory. There is a way that you can to test for TMB and HRP-streptavidin activity: Prepare a 100-fold dilution of HRP-Streptavidin...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"