概述

  • 产品名称
    人CD62E ELISA试剂盒(Selectin)
    参阅全部 CD62E 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 30 pg/ml
  • 范围
    24.69 pg/ml - 18000 pg/ml
  • 回收率

    94 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 94.39 83% - 103%
    Serum 93.75 83% - 102%
    Plasma 95.38 84% - 105%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD62E (Selectin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62E in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CD62E coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100512.

  • Representative Standard Curve using ab100512.

实验方案

文献

This product has been referenced in:
  • Chitalia N  et al. Impact of vitamin D supplementation on arterial vasomotion, stiffness and endothelial biomarkers in chronic kidney disease patients. PLoS One 9:e91363 (2014). Read more (PubMed: 24646518) »

See 1 Publication for this product

客户评价及客户问答

Yes, I can confirm that EDTA, Heparin, or Citrate collected plasma can be used with the ab100512 CD62E Human ELISA Kit

Thank you for contacting us.

All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

I hope this information has been of help. If your require any further information please do not...

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Thank you for your inquiry and I apologize for the delay while I was awaiting more information from our testing laboratory. There is a way that you can to test for TMB and HRP-streptavidin activity: Prepare a 100-fold dilution of HRP-Streptavidin...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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