人CD44var ELISA试剂盒(v6) (ab119519)

概述

  • 产品名称
    人CD44var ELISA试剂盒(v6)
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 3%
    批次间
    样品 n Mean SD CV%
    Overall 8 4.2%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.05 ng/ml
  • 范围
    0.32 ng/ml - 10 ng/ml
  • 复检

    106 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 106 89% - 115%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD44var (v6) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD44var (v6) concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), and urine.

    CD44var (v6) specific antibodies have been precoated onto a 96-well plate. Standards and test samples are added to the wells and along with a CD44var (v6) HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the CD44var (v6) amount of sample captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 功能
    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • 组织特异性
    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • 序列相似性
    Contains 1 Link domain.
  • 结构域
    The lectin-like LINK domain is responsible for hyaluronan binding.
  • 翻译后修饰
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 别名
    • CD44
    • CD44 antigen
    • CD44 molecule
    • CD44_HUMAN
    • CDw44
    • Cell surface glycoprotein CD44
    • Chondroitin sulfate proteoglycan 8
    • CSPG8
    • ECMR-III
    • Epican
    • Extracellular matrix receptor III
    • GP90 lymphocyte homing/adhesion receptor
    • Hematopoietic cell E and L selectin ligand
    • Heparan sulfate proteoglycan
    • Hermes antigen
    • Homing function and Indian blood group system
    • HUTCH-I
    • Hyaluronate receptor
    • IN
    • Indian blood group
    • LHR
    • MC56
    • MDU2
    • MDU3
    • MIC4
    • PGP-1
    • PGP-I
    • Pgp1
    • Phagocytic glycoprotein 1
    • Phagocytic glycoprotein I
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD44var ELISA Kit (v6) 图像

  • Representative Standard Curve using ab119519.

实验方案

Human CD44var ELISA Kit (v6) (ab119519)参考文献

ab119519 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

After reading the protocol I am sure that the whole plate is coated with the antibody as all the reagents are designed to be sufficient for 12 stripes. As mentioned in the protocol, the 32 wells are just an examp...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册