人CD40L ELISA试剂盒(ab99991)

概述

  • 产品名称
    人CD40L ELISA试剂盒
    参阅全部 CD40L 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 6 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 96.35 86% - 105%
    Serum 93.57 83% - 103%
    Plasma 94.63 84% - 105%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Human CD40L ELISA Kit (ab99991) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40L in serum, plasma (EDTA or heparin), and cell culture supernatants.


    This assay employs an antibody specific for Human CD40L coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD40L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human CD40 Ligand ELISA Kit (TNFSF5) (ab196268) from our SimpleStep ELISA® range.


     

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 功能
    Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • 组织特异性
    Specifically expressed on activated CD4+ T-lymphocytes.
  • 疾病相关
    Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • 序列相似性
    Belongs to the tumor necrosis factor family.
  • 翻译后修饰
    The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 别名
    • CD154
    • CD40 antigen ligand
    • CD40 ligand
    • CD40-L
    • CD40L
    • CD40L_HUMAN
    • CD40LG
    • gp39
    • hCD40L
    • HIGM1
    • IGM
    • IMD3
    • soluble form
    • T B cell activating molecule
    • T BAM
    • T cell antigen Gp39
    • T-cell antigen Gp39
    • TNF related activation protein
    • TNF-related activation protein
    • TNFSF5
    • TrAP
    • Tumor necrosis factor (ligand) superfamily member 5
    • Tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
    • Tumor necrosis factor ligand superfamily member 5
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab99991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD40L ELISA Kit 图像

  • Representative Standard Curve using ab99991.

  • Representative Standard Curve using ab99991.

实验方案

Human CD40L ELISA Kit (ab99991)参考文献

ab99991 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. This kit does recognize the extracellular region of CD40L (aa 47 – 261). Thus, it is used for the detection of soluble CD40L. The exact cross-reactivity of membrane-bound CD40L has not been tested. Please let me know if you...

Read More

Merci de votre patience.
Le laboratoire m'avait envoyé les données qu'ils ont obtenu avec ce lot. Veuillez les trouves dans le document attaché. Les résultats sont mieux que ceux que vous avez obtenue avec le kit que nous vous avions envoyé. Si vo...

Read More

Thank you for contacting us. We unfortunately can’t provide aspecific starting dilution factorat whichthere will be no interference from other plasma proteins in the assay.We recommend trying a series of dilutions until a linear response is measu...

Read More

Thank you for your reply. As I mentioned in my previous email, we do not have a recommended minimum dilution for this kit because the amount of dilution necessary will depend on your samples.  Our recommended starting dilution for further optimization ...

Read More

Thank you for contacting us.  Matrix effects can sometimes occur when certain factors in the plasma such as auto-antibodies, binding proteins, albumin, etc. can interfere with the assay if too much is present in your samples.  The result is that the sa...

Read More

Merci de nous avoir contactés. J'ai reçu les informations suivantes concernant les kits ELISA CD40L et PF4 : ab99991 doit être utilisé avec des échantillons de plasma traités EDTA ou héparine. L'utilisation d'autres anticoagulants n'est pas recomma...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册