概述

  • 产品名称人CD40 ELISA试剂盒
    参阅全部 CD40 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 5.5%
    批次间
    样品 n Mean SD CV%
    Overall 8 7%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    1.3 pg/ml
  • 范围
    7.8 pg/ml - 500 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 94 81% - 110%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD40 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD40 concentrations in cell culture supernatant, serum, plasma (EDTA, heparin) and amniotic fluid.

    CD40 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD40 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD40 sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
  • 别名
    • AI326936
    • B cell associated molecule
    • B cell associated molecule CD40
    • B cell surface antigen CD40
    • Bp50
    • CD 40
    • CD40 antigen
    • CD40 antigen (TNF receptor superfamily member 5)
    • CD40 molecule
    • CD40 molecule TNF receptor superfamily member 5
    • CD40 protein
    • CD40 type II isoform
    • CD40L receptor
    • CDw40
    • GP39
    • HIGM1
    • IGM
    • MGC9013
    • Nerve growth factor receptor related B lymphocyte activation molecule
    • NFRSF5
    • OTTHUMP00000031699
    • OTTHUMP00000031700
    • p50
    • T BAM
    • TBAM
    • TNF receptor superfamily member 5
    • TNFRSF5
    • TRAP
    • Tumor necrosis factor receptor superfamily member 5
    • Tumor necrosis factor receptor superfamily member 5 precursor
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD40 ELISA Kit 图像

  • Representative Standard Curve using ab119516.

实验方案

Human CD40 ELISA Kit (ab119516)参考文献

ab119516 has not yet been referenced specifically in any publications.

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