人CD28 ELISA试剂盒(ab119514)

概述

  • 产品名称人CD28 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.2%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.18 ng/ml
  • 范围
    0.78 ng/ml - 50 ng/ml
  • 复检

    117 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 105 90% - 130%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD28 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD28 concentrations in cell culture supernatant and serum.

    CD28 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD28 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD28 sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • 组织特异性Expressed in T-cells and plasma cells, but not in less mature B-cells.
  • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CD28
    • CD28 antigen
    • CD28 molecule
    • CD28_HUMAN
    • T-cell-specific surface glycoprotein CD28
    • TP44
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD28 ELISA Kit 图像

  • Representative Standard Curve using ab119514.

Human CD28 ELISA Kit (ab119514)参考文献

ab119514 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"