人CD26 ELISA试剂盒(ab119513)

概述

  • 产品名称人CD26 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 4.6%
    批次间
    样品 n Mean SD CV%
    Overall 8 9.1%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    7.3 ng/ml
  • 范围
    15.6 ng/ml - 500 ng/ml
  • 复检

    101 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 101 81% - 116%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD26 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD26 concentrations in cell culture supernatant and serum.

    CD26 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD26 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD26 sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
  • 组织特异性Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
  • 序列相似性Belongs to the peptidase S9B family. DPPIV subfamily.
  • 结构域The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.
  • 翻译后修饰The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
    N- and O-Glycosylated.
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
  • 细胞定位Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.
  • Information by UniProt
  • 别名
    • CD26 antigen
    • ADA-binding protein
    • ADABP
    • ADCP 2
    • ADCP-2
    • ADCP2
    • Adenosine deaminase complexing protein 2
    • CD 26
    • CD26
    • CD26 antigen 3
    • Dipeptidyl peptidase 4
    • Dipeptidyl peptidase 4 soluble form
    • Dipeptidyl peptidase IV
    • Dipeptidyl peptidase IV membrane form
    • Dipeptidyl peptidase IV soluble form
    • Dipeptidyl peptidase, intestinal
    • Dipeptidylpeptidase 4
    • Dipeptidylpeptidase IV
    • Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
    • DPP 4
    • DPP IV
    • DPP IV estoenzyme
    • DPP4
    • DPP4_HUMAN
    • DPPIV
    • Intestinal dipeptidyl peptidase
    • T cell activation antigen CD26
    • T-cell activation antigen CD26
    • TP 103
    • TP103
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD26 ELISA Kit 图像

  • Representative Standard Curve using ab119513.

实验方案

Human CD26 ELISA Kit (ab119513)参考文献

ab119513 has not yet been referenced specifically in any publications.

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