概述

  • 产品名称人CD23 ELISA试剂盒
    参阅全部 CD23 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4%
    批次间
    样品 n Mean SD CV%
    Overall 6.3%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    6.8 U/ml
  • 范围
    12.5 U/ml - 400 U/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 93 81% - 120%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD23 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD23 concentrations in cell culture supernatant and serum and plasma (EDTA, citrate, heparin).

    CD23 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD23 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD23 sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
  • 序列相似性Contains 1 C-type lectin domain.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Cell membrane. Secreted. Also exists as a soluble excreted form.
  • Information by UniProt
  • 别名
    • BLAST 2
    • BLAST-2
    • C-type lectin domain family 4 member J
    • CD23
    • CD23 antigen
    • CD23A
    • CLEC4J
    • Fc fragment of IgE low affinity II receptor for (CD23)
    • Fc-epsilon-RII
    • FCE2
    • Fcer2
    • FCER2_HUMAN
    • IGEBF
    • Immunoglobulin E-binding factor
    • Low affinity immunoglobulin epsilon Fc receptor
    • Low affinity immunoglobulin epsilon Fc receptor soluble form
    • Lymphocyte IgE receptor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD23 ELISA Kit 图像

  • Representative Standard Curve using ab119512.

实验方案

Human CD23 ELISA Kit (ab119512)参考文献

ab119512 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119512.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"