概述

  • 产品名称
    人CD23 ELISA试剂盒
    参阅全部 CD23 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4%
    批次间
    样品 n Mean SD CV%
    Overall 6.3%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    6.8 U/ml
  • 范围
    12.5 U/ml - 400 U/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 93 81% - 120%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD23 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD23 concentrations in cell culture supernatant and serum and plasma (EDTA, citrate, heparin).

    CD23 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD23 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD23 sample captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119512.

实验方案

文献

ab119512 has not yet been referenced specifically in any publications.

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