概述

  • 产品名称人CD14 ELISA试剂盒
    参阅全部 CD14 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 8 210.5ng/ml 2.6 1.23%
    Sample B 8 23.4ng/ml 1.14 4.6%
    批次间
    样品 n Mean SD CV%
    Sample A 8 210.5ng/ml 1.55 0.73%
    Sample B 8 24 2 8.3%
  • 测试
    2 x 96 well plate
  • 样品类型
    Cell culture supernatant, Milk, Serum, Plasma, Cell Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 6 ng/ml
  • 范围
    6.8 ng/ml - 220 ng/ml
  • 检测时间
    3h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD14 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD14 in Human serum, plasma, buffered solutions or cell culture media.

    A monoclonal antibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD14 concentrations and unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD14 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD14 present in the samples.

    This kit will recognize both endogenous and recombinant Human CD14.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 组织特异性Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 序列相似性Contains 11 LRR (leucine-rich) repeats.
  • 翻译后修饰N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 别名
    • CD 14
    • CD_antigen=CD14
    • CD14
    • CD14 antigen
    • CD14 molecule
    • CD14_HUMAN
    • LPS-R
    • Mo2
    • Monocyte differentiation antigen CD14
    • Monocyte differentiation antigen CD14 urinary form
    • Monocyte differentiation antigen CD14, membrane-bound form
    • Myeloid cell specific leucine rich glycoprotein
    • Myeloid cell-specific leucine-rich glycoprotein
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab45523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD14 ELISA Kit 图像

  • Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted

  • hu CD14 measured in biologicals showing quantity (ng) per mL of tested sample

实验方案

Human CD14 ELISA Kit (ab45523)参考文献

This product has been referenced in:
  • Malherbe G  et al. Circulating biomarkers of immune activation distinguish viral suppression from nonsuppression in HAART-treated patients with advanced HIV-1 subtype C infection. Mediators Inflamm 2014:198413 (2014). Read more (PubMed: 24808634) »

See 1 Publication for this product

Product Wall


ab45523 should recognize soluble CD14, as it has been tested and is guaranteed to detect the protein in cell culture supernatant, in which only soluble CD14 would be present.

I am sorry for the delay in getting back to you.

As discussed in my previous email, the human CD14 ELISA kits ab99989 and ab45523 are both sandwich ELISA kits which bind CD14 to the surface of the plate and use another antibody to detect the ...

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Thank you for your enquiry. I can suggest the following procedure which can be used to lyse the THP cells ready for CD14 detection using this ELISA kit: Lysis Buffer : Tris 50mM Nacl 150mM 1%NP40 (Nonidet P40) pH=8.0 1-Collect the cells by ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"