概述

  • 产品名称
    人CD137 ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 7.1%
    批次间
    样品 n Mean SD CV%
    Overall 9.9%
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.2 ng/ml
  • 范围
    0.78 ng/ml - 50 ng/ml
  • 回收率

    90 %

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD137 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD137 concentrations in cell culture supernatant and serum.

    CD137 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD137 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD137 captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-Human CD137 polyclonal antibody 1 x 100µl
    Human CD137 monoclonal antibody coated microplate (12 x 8 wells) 1 unit
    Human CD137 Standard, lyophilized (100 ng/mL on reconstitution) 2 vials
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 功能
    Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation.
  • 组织特异性
    Expressed on the surface of activated T-cells.
  • 序列相似性
    Contains 4 TNFR-Cys repeats.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 别名
    • 4-1BB
    • 4-1BB ligand receptor
    • CD137
    • CD137 antigen
    • CDw137
    • Homolog of mouse 4 1BB
    • ILA
    • Induced by lymphocyte activation (ILA)
    • Interleukin activated receptor homolog of mouse Ly63
    • Receptor protein 4 1BB
    • T-cell antigen 4-1BB homolog
    • T-cell antigen ILA
    • TNFRSF9
    • TNR9_HUMAN
    • Tumor necrosis factor receptor superfamily member 9
    see all
  • 数据库链接

    应用

    Our Abpromise guarantee covers the use of ab119511 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent concentration.

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab119511.

    实验方案

    文献

    ab119511 has not yet been referenced specifically in any publications.

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