概述
- 产品名称人CD137 ELISA试剂盒
- 检测方法Colorimetric
- 精确度
批次内 样品 n Mean SD CV% Overall 7.1% 批次间 样品 n Mean SD CV% Overall 9.9% - 样品类型Cell culture supernatant, Serum
- 检测类型Sandwich (quantitative)
- 灵敏度0.2 ng/ml
- 范围0.78 ng/ml - 50 ng/ml
- 复检
90 %
- 实验步骤Multiple steps standard assay
- 种属反应性与反应: Human
- 产品概述
Abcam’s CD137 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD137 concentrations in cell culture supernatant and serum.
CD137 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD137 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD137 captured in plate.
- 经测试应用适用于: Sandwich ELISAmore details
- 平台Microplate
性能
- 存放说明Store at +4°C. Please refer to protocols.
-
组件 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-Human CD137 polyclonal antibody 1 x 100µl Human CD137 monoclonal antibody coated microplate (12 x 8 wells) 1 unit Human CD137 Standard, lyophilized (100 ng/mL on reconstitution) 2 vials Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml - 研究领域
- 功能Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation.
- 组织特异性Expressed on the surface of activated T-cells.
- 序列相似性Contains 4 TNFR-Cys repeats.
- 细胞定位Membrane.
- Information by UniProt
-
别名
- 4-1BB
- 4-1BB ligand receptor
- CD137
see all -
数据库链接
- Entrez Gene: 3604 Human
- Omim: 602250 Human
- SwissProt: Q07011 Human
- Unigene: 86447 Human
应用
Our Abpromise guarantee covers the use of ab119511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Human CD137 ELISA Kit 图像
-
Typical Standard CurveRepresentative Standard Curve using ab119511.
实验方案
Human CD137 ELISA Kit (ab119511)参考文献
ab119511 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"