人CD134 ELISA试剂盒(ab119510)

概述

  • 产品名称人CD134 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8%
    批次间
    样品 n Mean SD CV%
    Overall 12%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    1.8 pg/ml
  • 范围
    78 pg/ml - 5000 pg/ml
  • 复检

    88 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD134 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD134 concentrations in cell culture supernatant and serum.

    CD134 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated CD134 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD134 captured on the plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Receptor for TNFSF4/OX40L/GP34.
  • 序列相似性Contains 4 TNFR-Cys repeats.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • ACT35
    • ACT35 antigen
    • CD134
    • CD134 antigen
    • Lymphoid activation antigene ACT35
    • OX40
    • OX40 antigen
    • OX40 cell surface antigen
    • OX40 homologue
    • OX40L receptor
    • TAX transcriptionally-activated glycoprotein 1 receptor
    • TNFRSF4
    • TNR4_HUMAN
    • Tumor necrosis factor receptor superfamily member 4
    • TXGP1L
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD134 ELISA Kit 图像

  • Representative Standard Curve using ab119510.

实验方案

Human CD134 ELISA Kit (ab119510)参考文献

ab119510 has not yet been referenced specifically in any publications.

Product Wall

Unfortunately, the antibodies used in ab119510 ELISA kit have not had their epitopes mapped and thus we are not able to provide you with the binding region.

Thank you for contacting Abcam.

We use different antibodies for this ELISA. Unfortunately which ones we use is proprietary and so I cannot provide anymore information.
If there is anything else I can help you with, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"