概述

  • 产品名称人CD130 ELISA试剂盒(GP130)
    参阅全部 CD130 (gp130) 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 8 1751pg/ml 17.61 1%
    B 8 264.3pg/ml 8.67 3.27%
    批次间
    样品 n Mean SD CV%
    A 16 1782pg/ml 26.64 1.49%
    B 16 247pg/ml 11.99 4.85%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    56.25 pg/ml - 1800 pg/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 97 % - %

  • 检测时间
    1h 40m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD130 (GP130) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD130 in Human serum, plasma, buffered solutions and supernatants.

    A monoclonal antibody specific for CD130 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD130 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD130 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD130 present in the samples.

    This kit will recognize both endogenous and recombinant Human CD130.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
  • 细胞定位Cell membrane; single-pass type I membrane protein and secreted
  • 别名
    • CD130 antigen
    • CDw130
    • gp130
    • IL6 R beta
    • Interleukin 6 receptor subunit beta
    • Interleukin 6 signal transducer
    • Membrane glycoprotein 130
    • Oncostatin M receptor alpha subunit
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human CD130 ELISA Kit (GP130) 图像

  •  Representative Standard Curve using ab46135

实验方案

Human CD130 ELISA Kit (GP130) (ab46135)参考文献

ab46135 has not yet been referenced specifically in any publications.

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