概述

  • 产品名称
    人CD130 ELISA试剂盒(GP130)
    参阅全部 CD130 (gp130) 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 8 1751pg/ml 17.61 1%
    B 8 264.3pg/ml 8.67 3.27%
    批次间
    样品 n Mean SD CV%
    A 16 1782pg/ml 26.64 1.49%
    B 16 247pg/ml 11.99 4.85%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    56.25 pg/ml - 1800 pg/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 97 % - %

  • 检测时间
    1h 40m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s CD130 (GP130) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD130 in Human serum, plasma, buffered solutions and supernatants.

    A monoclonal antibody specific for CD130 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD130 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD130 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD130 present in the samples.

    This kit will recognize both endogenous and recombinant Human CD130.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 2 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-CD130 Red 1 x 400µl 2 x 400µl
    CD130 Microplate (12 x 8 well strips) 1 unit 2 units
    CD130 Standard (lyophilized) Yellow 2 vials 4 vials
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 相关性
    CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
  • 细胞定位
    Cell membrane; single-pass type I membrane protein and secreted
  • 别名
    • CD130 antigen
    • CDw130
    • gp130
    • IL6 R beta
    • Interleukin 6 receptor subunit beta
    • Interleukin 6 signal transducer
    • Membrane glycoprotein 130
    • Oncostatin M receptor alpha subunit
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  •  Representative Standard Curve using ab46135

实验方案

文献

ab46135 has not yet been referenced specifically in any publications.

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