人CCL28 ELISA试剂盒(ab99988)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 27.4 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人CCL28 ELISA试剂盒
参阅全部 CCL28/MEC 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 25 pg/ml -
范围
27.4 pg/ml - 20000 pg/ml -
回收率
> 65 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 67.7 59% - 81% Serum 70.6 60% - 98% Plasma 68.6 59% - 82% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s CCL28 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL28 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CCL28 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman CCL28 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CCL28 2 vials CCL28 Microplate (12 strips x 8 wells) 1 x 200µl Recombinant Human CCL28 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. -
组织特异性
Preferentially expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. Also found in prostate, spleen, thyroid, psoriasis skin and in lower levels in peripheral blood leukocytes, small intestine, Peyer patches, stomach and normal skin. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
细胞定位
Secreted. - Information by UniProt
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别名
- C C motif chemokine ligand 28
- C-C motif chemokine 28
- CC chemokine CCL28
see all -
数据库链接
- Entrez Gene: 56477 Human
- Omim: 605240 Human
- SwissProt: Q9NRJ3 Human
- Unigene: 656904 Human
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab99988 被引用在 2 文献中.
- Huang G et al. Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells. Sci Rep 6:27152 (2016). PubMed: 27250766
- Yoon JH et al. Plasma CC-chemokine ligand 28 level is correlated with hematopoietic stem cells in human cord blood. Transfusion 55:1008-12 (2015). PubMed: 25412854