人Cathepsin L ELISA试剂盒(ab119509)

概述

  • 产品名称人Cathepsin L ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 6.2%
    批次间
    样品 n Mean SD CV%
    Overall 6%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    1.7 ng/ml
  • 范围
    3.1 ng/ml - 50 ng/ml
  • 复检

    92 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 92 78% - 113%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Cathepsin L Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cathepsin L concentrations in cell culture supernatant and serum. This kit is not suitable for the detection of Cathepsin L in plasma.

    Cathepsin L specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Cathepsin L detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cathepsin L captured on the plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Cathepsin L ELISA Kit 图像

  • Representative Standard Curve using ab119509.

实验方案

Human Cathepsin L ELISA Kit (ab119509)参考文献

ab119509 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your telephone enquiry.

I can confirm as discussed on the telephone that we have the following cathepsin L ELISA kit

ab119509Cathepsin L Human ELISA Kit (with color giving dyes
http://www.abcam.com/index.html?da...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"