概述

  • 产品名称人Cathepsin B ELISA试剂盒
    参阅全部 Cathepsin B 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    156 pg/ml - 10000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human Cathepsin B in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cathepsin B in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Cathepsin B specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cathepsin B amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • 序列相似性Belongs to the peptidase C1 family.
  • 细胞定位Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 别名
    • Amyloid precursor protein secretase
    • APP secretase
    • APPS
    • CATB_HUMAN
    • Cathepsin B heavy chain
    • Cathepsin B1
    • CathepsinB
    • CPSB
    • CTSB
    • cysteine protease
    • OTTHUMP00000116009
    • OTTHUMP00000229510
    • OTTHUMP00000229511
    • OTTHUMP00000229512
    • OTTHUMP00000229514
    • OTTHUMP00000229515
    • OTTHUMP00000229516
    • Preprocathepsin B
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Cathepsin B ELISA Kit 图像

  • Cathepsin B measured in culture supernatants from control (-) or stimulated cells cells (P/L: 24 hours with 50 ng x mL-1 of PMA (ab120297) with 1 ug x mL-1 of LPS (Sigma) for the last 6 hours; PHA: 48 hours with 2% PHA-M; LifeTechnologies). Data shown represents duplicates (+/- SD), samples tested in the dilution range of 1/1-1/20.

  • Cathepsin B measured in biological fluids (tested in the range of 1/10-1/1000; duplicates +/- SD).

  • Representative Standard Curve Using ab119584.

实验方案

Human Cathepsin B ELISA Kit (ab119584)参考文献

This product has been referenced in:
  • Zhu S  et al. Inhibiting autophagy potentiates the anticancer activity of IFN1@/IFNa in chronic myeloid leukemia cells. Autophagy 9:317-27 (2013). Read more (PubMed: 23242206) »

See 1 Publication for this product

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J’ai eu une réponse du laboratoire qui m’a confirmé que les plaques sont divisibles. La plaque peut être divisé en 12 donc vous pouvez tester 8 échantillons à chaque fois.

Une foi...

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Thank you for your reply which has been forwarded to me as my colleague Karin is currently away from the office.

I am very sorry to confirm that there are no discounts available that I can provide on this occasion. I can let you know that if...

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Thank you for contacting us and sorry for the delay in getting back to you.

I can confirm that the Cathepsin B Human ELISA Kit (ab119584) can detect both the mature form and uncleaved form of Cathepasin B.

The kit has been found to ...

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