人Caspase-9 ELISA试剂盒(ab119508)

概述

  • 产品名称人Caspase-9 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 6.6%
    批次间
    样品 n Mean SD CV%
    Overall 8 9%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Cell Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.4 ng/ml
  • 范围
    1.6 ng/ml - 100 ng/ml
  • 复检

    103 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Caspase-9 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Caspase-9 concentrations in cell lysate, cell culture supernatant and serum.

    Caspase-9 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Caspase-9 detection antibody and the microplate is then incubated at room temperature. Following washing a HRP conjugated anti-rabbit-IgG antibody is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Caspase-9 amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • 组织特异性Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • 序列相似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 发展阶段Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • 翻译后修饰Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt
  • 别名
    • APAF-3
    • APAF3
    • Apoptotic protease Mch-6
    • Apoptotic protease-activating factor 3
    • CASP-9
    • CASP9
    • CASP9_HUMAN
    • Caspase 9 apoptosis related cysteine peptidase
    • Caspase-9 subunit p10
    • ICE-LAP6
    • ICE-like apoptotic protease 6
    • MCH6
    • PPP1R56
    • Protein phosphatase 1 regulatory subunit 56
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Caspase-9 ELISA Kit 图像

  • Representative Standard Curve using ab119508.

实验方案

Human Caspase-9 ELISA Kit (ab119508)参考文献

ab119508 has not yet been referenced specifically in any publications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"