人Carcino Embryonic Antigen ELISA试剂盒(CEA) (ab99992)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.343 ng/ml - 250 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Carcino Embryonic Antigen ELISA试剂盒(CEA) -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.2 ng/ml -
范围
0.343 ng/ml - 250 ng/ml -
回收率
> 100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 116.7 93% - 136% Serum 145.6 135% - 156% Plasma 103.8 82% - 124% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Carcinoembryonic Antigen (CEA) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEA in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human Carcinoembryonic Antigen ELISA Kit (CD66e) (ab183365) from our SimpleStep ELISA® range.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 1,000X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CEA 2 vials CEA Microplate (12 x 8 well strips) 1 unit Recombinant Human CEA Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性
Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). -
细胞定位
Cell Membrane -
别名
- Carcinoembryonic antigen
- Carcinoembryonic antigen related cell adhesion molecule 5
- CD66e
see all -
数据库链接
- Entrez Gene: 1048 Human
- SwissProt: P06731 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab99992 被引用在 11 文献中.
- Ma J et al. Detection Rate and Prognosis of Lymph Nodes in Gastric Cancer Using Nano Carbon Combined with In Vitro Anatomical Sorting. Comput Intell Neurosci 2022:4540176 (2022). PubMed: 36052030
- Joshi S et al. Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors. Sci Rep 12:15315 (2022). PubMed: 36097151
- Alhetheel A et al. Differential expression of carcinoembryonic antigen-related cell adhesion molecule-5 (CEACAM5) and dipeptidyl peptidase-4 (DPP4) with detection of Middle East respiratory syndrome-coronavirus in peripheral blood. J Infect Public Health 15:1315-1320 (2022). PubMed: 36279687
- Kang C et al. Construction and Validation of a Lung Cancer Diagnostic Model Based on 6-Gene Methylation Frequency in Blood, Clinical Features, and Serum Tumor Markers. Comput Math Methods Med 2021:9987067 (2021). PubMed: 34257703
- Gökçay Canpolat A et al. Calcitonin levels in washout samples vs. cytology in the detection of malignant lymph node metastasis in recurrent medullary thyroid cancer. Turk J Med Sci 51:3061-3066 (2021). PubMed: 34519193