人Anti-Candida albicans IgG ELISA试剂盒(ab108715)

概述

  • 产品名称
    人Anti-Candida albicans IgG ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Pos.Serum 8 4.3%
    批次间
    样品 n Mean SD CV%
    Pos.Serum 20 5.1%
  • 样品类型
    Serum, Plasma
  • 检测类型
    Indirect
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s anti-Candida albicans IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Candida albicans in Human serum and plasma.

     

    A 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgG sample captured in plate.

  • 经测试应用
    适用于: Indirect ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA Use at an assay dependent concentration.

实验方案

文献

ab108715 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Plasma samples were all diluted 1:100 as suggested in the manual. All other steps were performed according to the instructions.
This ELISA is very fast (pre-coated wells and no blocking step required), however one has to keep in mind that it does not provide quantitative data. The optical densities of the samples are proportionate to the anti-C.albicans IgG levels in the samples. A positive control, a negative control and a cut-off control are provided in order to check the ELISA is working.
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提交于 Jul 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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