人C Reactive Protein ELISA试剂盒(PTX1) (ab108826)

概述

  • 产品名称人C Reactive Protein ELISA试剂盒(PTX1)
    参阅全部 C Reactive Protein 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.25 ng/ml
  • 范围
    0.5 ng/ml - 5 ng/ml
  • 复检

    98.5 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s C Reactive Protein (PTX1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein concentrations in Human plasma, serum, saliva, milk, urine and cell culture supernatants.

    A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • 组织特异性Found in plasma.
  • 序列相似性Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Pentraxin 1, short
    • C reactive protein
    • C reactive protein pentraxin related
    • C-reactive protein(1-205)
    • CRP
    • CRP_HUMAN
    • MGC88244
    • Pentraxin 1
    • PTX 1
    • PTX1
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab108826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human C Reactive Protein ELISA Kit (PTX1) 图像

  • Representative Standard Curve using ab108826

实验方案

Human C Reactive Protein ELISA Kit (PTX1) (ab108826)参考文献

This product has been referenced in:
  • Strøm Halden TA  et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014). Read more (PubMed: 24452849) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

1. Your software may have a way to adjust the correction wavelength, or you can run it without the correction factor. The 570 wave length is subtracted to reduce the background noise and lower the OD. If you are unabl...

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I checked the details and can confirm that C Reactive Protein (PTX1) Human ELISA Kit ab108826 has been tested and covered by the guarantee for use in urine samples. Our apologies for any confusion.

I was not able to find any literature to...

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Thank you for your enquiry regarding these kits.

I would suggest reading through the Protocol Booklets for each of these kits provided on the product datasheets. These documents contain specific information about the sample types, sample prep...

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Thank you for your enquiry. I can confirm that both these kits ab46042 and ab108826 should be suitable for use with saliva samples. I can confirm that  kit ab108826 has been tested on saliva samples,  We suggest to use saliva samples at 1x (no d...

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