人C Reactive蛋白ELISA试剂盒(PTX1) (ab108826)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.25 ng/ml - 16 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人C Reactive蛋白ELISA试剂盒(PTX1)
参阅全部 C Reactive Protein 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4% 批次间 样品 n Mean SD CV% Overall 11.3% -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.2 ng/ml -
范围
0.25 ng/ml - 16 ng/ml -
回收率
96 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s C Reactive Protein (PTX1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C Reactive Protein concentrations in Human plasma, serum, saliva, milk, urine and cell culture supernatants.
A C Reactive Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a C Reactive Protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of C Reactive Protein captured in plate.
Get results in 90 minutes with Human C Reactive Protein ELISA Kit (ab181416) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human C Reactive Protein Antibody 1 x 120µl C Reactive Protein Microplate (12 x 8 well strips) 1 unit C Reactive Protein Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
研究领域
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功能
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
组织特异性
Found in plasma. -
序列相似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
细胞定位
Secreted. - Information by UniProt
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别名
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
数据库链接
- Entrez Gene: 1401 Human
- Omim: 123260 Human
- SwissProt: P02741 Human
- Unigene: 709456 Human
- Unigene: 76452 Human
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108826 被引用在 1 文献中.
- Strøm Halden TA et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014). PubMed: 24452849