样品 | n | Mean | SD | CV% |
---|---|---|---|---|
Sample A | 8 | 5.629ng/ml | 0.34 | 6% |
Sample B | 8 | 1.231ng/ml | 0.06 | 4.8% |
样品 | n | Mean | SD | CV% |
---|---|---|---|---|
Sample A | 8 | 5.44ng/ml | 0.43 | 7.9% |
Sample B | 8 | 1.314ng/ml | 0.1 | 7.6% |
Abcam’s c-Kit Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of c-Kit in Human serum, plasma, buffered solutions or supernatants.
A monoclonal antibody specific for c-Kit has been coated onto the wells of the microtiter strips provided. Samples, including standards of known c-Kit concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for c-Kit are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of c-Kit present in the samples.
This kit will recognize both endogenous and recombinant Human c-Kit.
组件 | 标识符 | 1 x 96 tests | 2 x 96 tests |
---|---|---|---|
10X Standard Diluent Buffer | Black | 1 x 25ml | 1 x 25ml |
200X Wash Buffer | White | 1 x 10ml | 2 x 10ml |
Biotinylated anti c-Kit | 1 x 0.4ml | 2 x 0.4ml | |
Biotinylated Antibody Diluent | Red | 1 x 7.5ml | 1 x 13ml |
Chromogen TMB Substrate Solution | 1 x 11ml | 1 x 24ml | |
c-Kit Control | Silver | 2 vials | 4 vials |
c-Kit Microplate (12 x 8 well strips) | 1 unit | 2 units | |
c-Kit Standard (Lyophilized) | Yellow | 2 vials | 4 vials |
HRP Diluent | Red | 1 x 23ml | 1 x 23ml |
Plastic Plate Covers | 1 x 2 units | 1 x 2 units | |
Stop Reagent | Black | 1 x 11ml | 2 x 11ml |
Streptavidin-HRP | 2 x 5µl | 4 x 5µl |
Our Abpromise guarantee covers the use of ab45924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
ab45924 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"