人c-Kit ELISA试剂盒(CD117) (ab45924)

概述

  • 产品名称人c-Kit ELISA试剂盒(CD117)
    参阅全部 c-Kit 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Sample A 8 5.629ng/ml 0.34 6%
    Sample B 8 1.231ng/ml 0.06 4.8%
    批次间
    样品 n Mean SD CV%
    Sample A 8 5.44ng/ml 0.43 7.9%
    Sample B 8 1.314ng/ml 0.1 7.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.095 ng/ml
  • 范围
    0.312 ng/ml - 10 ng/ml
  • 检测时间
    1h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s c-Kit Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of c-Kit in Human serum, plasma, buffered solutions or supernatants.

    A monoclonal antibody specific for c-Kit has been coated onto the wells of the microtiter strips provided. Samples, including standards of known c-Kit concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for c-Kit are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of c-Kit present in the samples.

    This kit will recognize both endogenous and recombinant Human c-Kit.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性KIT encodes the human homolog of the proto-oncogene c-kit. c-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
  • 细胞定位Cell Membrane; single-pass type I membrane protein
  • 别名
    • CD117
    • Mast/stem cell growth factor receptor precursor
    • PBT
    • Proto oncogene tyrosine protein kinase Kit
    • SCFR
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab45924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human c-Kit ELISA Kit (CD117) 图像

  • Representative Standard Curve using ab45924.

实验方案

Human c-Kit ELISA Kit (CD117) (ab45924)参考文献

ab45924 has not yet been referenced specifically in any publications.

Product Wall


The c-Kit Human ELISA Kit (ab45924) and Ferritin Human ELISA Kit (ab108698) both come with strip wells provided.

Thank you for your enquiry.

ab45925 is suitable for Cerebrospinal fluid.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"