概述

  • 产品名称人BSG ELISA试剂盒(CD147)
    参阅全部 CD147 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    31.2 pg/ml - 2000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human BSG (CD147) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BSG in cell culture supernatants, serum, (heparin, EDTA), saliva, urine and Human milk.

    A BSG specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BSG is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BSG amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
  • 组织特异性Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
  • 序列相似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 别名
    • 5F7
    • BASI_HUMAN
    • Basigin
    • Bsg
    • CD147
    • Collagenase stimulatory factor
    • EMMPRIN
    • Extracellular matrix metalloproteinase inducer
    • Leukocyte activation antigen M6
    • OK blood group antigen
    • TCSF
    • Tumor cell-derived collagenase stimulatory factor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human BSG ELISA Kit (CD147) 图像

  • Representative Standard Curve using ab119592

实验方案

Human BSG ELISA Kit (CD147) (ab119592)参考文献

ab119592 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119592.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"