概述

  • 产品名称
    人Anti-Brucella IgG ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Pos.Serum 23 5.3%
    Pos.Serum 24 4.2%
    批次间
    样品 n Mean SD CV%
    Pos.Serum 12 3.6%
    Pos.Serum 12 4.8%
  • 样品类型
    Serum, Plasma
  • 检测类型
    Indirect
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s anti-Brucella IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Brucella in Human serum and plasma.

     

    A 96-well plate has been precoated with Brucella antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Brucella-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Brucella IgG sample captured in plate.

  • 经测试应用
    适用于: Indirect ELISAmore details
  • 平台
    Microplate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab108712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA Use at an assay dependent dilution.

实验方案

文献

This product has been referenced in:
  • Zhu J  et al. Lysine methylation represses p53 activity in teratocarcinoma cancer cells. Proc Natl Acad Sci U S A 113:9822-7 (2016). WB ; Human . Read more (PubMed: 27535933) »

See 1 Publication for this product

客户评价及客户问答

The positive and negative IgG controls in the assay are in fact yellow solutions (not the vials), due to the addition of the preservative KathonTM, also known generically as methylisothiazolinone. The concentration of the preservative is 0.1% w/v.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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