人breast组织裂解液(ductal carcinoma) - soluble fraction (female, 47 years) (ab43536)

概述

  • 产品名称人breast组织裂解液(ductal carcinoma) - soluble fraction (female, 47 years)
    参阅全部 Breast 细胞裂解液
  • 描述
    人breast soluble fraction裂解液
  • 常规说明

    Tissue: Breast Location: Left breast. Diagnosis: Infiltrating ductal carcinoma. Stage: IIIA T3N1M0 Grade: I Gross findings: Tumor size 6 cm., ill demarcated. Cut section firm and white. Ipsilateral axillary lymph nodes examined: 10 Microscopic findings: The tumor is composed of proliferation of malignant epithelial cells. The epithelial cells grown in the enlarged ducts contained comedo-necroses. The tumor cells have irregular, basophilic nuclei and abundant cytoplasm. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are abundant. Some areas show micro-invasions. The stroma is infiltrated by numerous inflammatory cells. The lymph nodes show metastasis (6/10).

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43536: Extraction 1: soluble protein fraction ab43536 Human Breast Tumor Tissue lysate ab43535 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43538 Human Breast Tumor Tissue lysate ab43537 Human Breast Normal Tissue lysate (matched)

性能

  • 形式Liquid
  • 存放说明Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Constituents: PBS, 0.0001% Pepstatin A, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.017% PMSF, 0.1% SDS, 0.029% EDTA
  • Concentration information loading...
  • 纯化说明Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究领域

    Human breast tissue lysate (ductal carcinoma) - soluble fraction (female, 47 years) (ab43536)参考文献

    ab43536 has not yet been referenced specifically in any publications.

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