人Anti-Borrelia burgdorferi IgM ELISA试剂盒(ab108711)

概述

  • 产品名称人Anti-Borrelia burgdorferi IgM ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Pos. Serum 8 8.6%
    批次间
    样品 n Mean SD CV%
    Pos. Serum 14 4.1%
  • 测试
    1 x 96 test
  • 样品类型
    Serum, Plasma
  • 检测类型Indirect
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s anti-Borrelia burgdorferi IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF (cerebrospinal fluid).

     

    A 96-well plate has been precoated with recombinant Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgM sample captured in plate.

  • 经测试应用适用于: Indirect ELISAmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab108711 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Indirect ELISA Use at an assay dependent dilution.

    实验方案

    Human Anti-Borrelia burgdorferi IgM ELISA Kit (ab108711)参考文献

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