概述

  • 产品名称
    人BNP ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    14 pg/ml
  • 范围
    14 pg/ml - 1000 pg/ml
  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Serum 78.12 68% - 83%
    Plasma 80.96 67% - 107%
    Cell culture media 94.31 83% - 105%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s BNP Human ELISA Kit (ab193694) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BNP in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human BNP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures BNP present in the samples. The wells are washed and biotinylated anti-Human BNP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of BNP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Pre-coated microplate (12 x 8 well strips)

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated Human BNP Detection Antibody (lyophilized) 2 vials
    Human BNP Standards 2 vials
    Pre-coated Human BNP Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 功能
    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • 组织特异性
    Brain and also in atria, but at much lower levels than ANP.
  • 序列相似性
    Belongs to the natriuretic peptide family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • ANFB_HUMAN
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • Brain type natriuretic peptide
    • Gamma-brain natriuretic peptide
    • Natriuretic peptide B
    • Natriuretic peptide precursor B
    • Natriuretic peptides B
    • Natriuretic protein
    • nppb
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab193694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Standard curve using ab193694 BNP Human ELISA Kit.

    Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

实验方案

文献

ab193694 has not yet been referenced specifically in any publications.

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