概述

  • 产品名称人BMP6 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 150 pg/ml
  • 范围
    200 pg/ml - 20000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 93.88 84% - 107%
    Serum 96.53 85% - 104%
    Plasma 94.69 84% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s BMP6 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP6 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human BMP6 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BMP6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species. BMPs are involved in embryogenesis and morphogenesis of various tissues and organs. BMP6 is an autocrine stimulator of chondrocyte differentiation and has been implicated in the development of embryonic kidney and urinary systems. It is involved in liver growth and differentiation, keratinocyte differentiation, and regulation of neuronal tissue development. BMP6 expression is localized to smooth muscle cells of normal cerebral blood vessels, intimal plaques and atherosclerotically changed blood vessels, further suggesting a role as a regulator of developing tissues.
  • 别名
    • BMP 6
    • Bone morphogenetic protein 6
    • Vegetal related (TGFB related) cytokine
    • Vegetal related growth factor (TGFB related)
    • VG 1 R
    • VG 1 related protein
    • Vg1 related sequence
    • VGR
    • VGR 1
    • VGR1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human BMP6 ELISA Kit 图像

  • Representative standard curve using ab99984

实验方案

Human BMP6 ELISA Kit (ab99984)参考文献

ab99984 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99984.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"