人BMP5 ELISA试剂盒(ab119583)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人BMP5 ELISA试剂盒
参阅全部 BMP5 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 242pg/ml 16.45 = 6.8% 2 16 1573pg/ml 69.21 = 4.4% 3 16 4363pg/ml 314.13 = 7.2% 批次间 样品 n Mean SD CV% 1 24 266pg/ml 20.74 = 7.8% 2 24 1683pg/ml 82.46 = 4.9% 3 24 4290pg/ml 347.49 = 8.1% -
样品类型
Cell culture supernatant, Serum, Tissue -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
156 pg/ml - 10000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human BMP5 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BMP5 in cell culture supernatants, serum and bone tissue.
A BMP5 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BMP5 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BMP5 amount of sample captured in plate.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human BMP5 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human BMP5 antibody 1 x 100µl Lyophilized recombinant Human BMP5 standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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功能
Induces cartilage and bone formation. -
组织特异性
Expressed in the lung and liver. -
序列相似性
Belongs to the TGF-beta family. -
细胞定位
Secreted. - Information by UniProt
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别名
- BMP 5
- BMP-5
- Bmp5
see all -
数据库链接
- Entrez Gene: 653 Human
- Omim: 112265 Human
- SwissProt: P22003 Human
- Unigene: 296648 Human
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab119583 被引用在 1 文献中.
- Xie S et al. Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling. Int J Biol Sci 17:2367-2379 (2021). PubMed: 34239363