人Betacellulin ELISA试剂盒(ab189575)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人Betacellulin ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 5 3% 批次间 样品 n Mean SD CV% Serum 3 5% -
样品类型
Cell culture supernatant, Milk, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
15 pg/ml -
范围
31.3 pg/ml - 2000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Milk 83 82% - 85% Serum 104 103% - 106% Tissue Culture Media 101 99% - 103% Hep Plasma 99 98% - 101% EDTA Plasma 90 87% - 92% Cit plasma 94 91% - 100% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Betacellulin ELISA Kit (ab189575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Betacellulin protein in hep plasma, milk, serum, cit plasma, cell culture supernatant, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Betacellulin with 15 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
说明
Betacellulin (BTC) is an EGF growth factor family member that binds to EGF Receptor family members. BTC is expressed as an 178 amino acid precursor form that is a single-pass transmembrane protein (this form is called probetacellulin). Subsequent cleavage produces the soluble and secreted 80 amino acid betacellulin fragment from the extracellular portion of probetacellulin. This assay reacts with the soluble betacellulin protein. There is high BTC protein sequence similarity among mammals.
-
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human BTC Capture Antibody 1 x 600µl 10X Human BTC Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human BTC Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
-
相关性
The protein encoded by this gene is a member of the mammalian members of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This encoded protein is a ligand for the EGF receptor. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. -
细胞定位
Cell Membrane and Secreted; extracellular space. -
别名
- Betacellulin precursor
- BTC
- Probetacellulin
-
数据库链接
- Entrez Gene: 685 Human
- SwissProt: P35070 Human
图片
-
Background-subtracted data values (mean +/- SD) are graphed.
-
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
-
Serum from 10 apparently healthy male donors was measured in duplicate. Six of the donors tested below the lowest tested standard. The mean Betacellulin concentration of the four donors was determined to be 125 pg/mL with a range of 88-173 pg/mL.
-
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab189575 被引用在 2 文献中.
- Torrino S et al. UBTD1 regulates ceramide balance and endolysosomal positioning to coordinate EGFR signaling. Elife 10:N/A (2021). PubMed: 33884955
- Sawrey-Kubicek L et al. Whole egg consumption compared with yolk-free egg increases the cholesterol efflux capacity of high-density lipoproteins in overweight, postmenopausal women. Am J Clin Nutr 110:617-627 (2019). PubMed: 31172172