概述

描述

  • 性质
    Synthetic

技术指标

Our Abpromise guarantee covers the use of ab20775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Blocking - Blocking peptide for Anti-beta Tubulin antibody - Loading Control (ab6046), Anti-beta Tubulin antibody (ab21057), Anti-beta Tubulin antibody - Loading Control (HRP) (ab21058)

  • 形式
    Liquid
  • 补充说明

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

常规信息

  • 别名
    • Beta 4 tubulin
    • Beta 5 tubulin
    • beta Ib tubulin
    • Beta1 tubulin
    • Class I beta tubulin
    • M40
    • MGC117247
    • MGC16435
    • OK/SW cl.56
    • OK/SWcl.56
    • TBB5_HUMAN
    • TUBB
    • TUBB 1
    • TUBB 2
    • TUBB 5
    • TUBB1
    • TUBB2
    • TUBB5
    • tubulin beta 1 chain
    • Tubulin beta 2 chain
    • tubulin beta 5 chain
    • Tubulin beta chain
    • Tubulin beta class I
    • tubulin beta polypeptide
    • Tubulin beta-5 chain
    see all
  • 功能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 组织特异性
    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • 疾病相关
    Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • 序列相似性
    Belongs to the tubulin family.
  • 结构域
    The highly acidic C-terminal region may bind cations such as calcium.
  • 翻译后修饰
    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • 细胞定位
    Cytoplasm, cytoskeleton.
  • Information by UniProt

图片

文献

This product has been referenced in:
  • Zhao H  et al. A humanized pattern of aromatase expression is associated with mammary hyperplasia in mice. Endocrinology 153:2701-13 (2012). Read more (PubMed: 22508516) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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