概述

  • 产品名称
    人beta Actin多肽

描述

  • 性质
    Synthetic
  • 氨基酸序列
    • 种属
      Human

技术指标

Our Abpromise guarantee covers the use of ab13772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Blocking - Blocking peptide for Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224), Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226), Anti-beta Actin antibody (ab8227), Anti-beta Actin antibody - Loading Control (ab8229)

  • 形式
    Liquid
  • 补充说明

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

常规信息

  • 别名
    • A26C1A
    • A26C1B
    • ACTB
    • ACTB_HUMAN
    • Actin beta
    • Actin cytoplasmic 1
    • Actin, cytoplasmic 1, N-terminally processed
    • Actx
    • b actin
    • Beta cytoskeletal actin
    • Beta-actin
    • BRWS1
    • E430023M04Rik
    • MGC128179
    • PS1TP5 binding protein 1
    • PS1TP5BP1
    see all
  • 功能
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 疾病相关
    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • 序列相似性
    Belongs to the actin family.
  • 翻译后修饰
    ISGylated.
  • 细胞定位
    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

文献

This product has been referenced in:
  • Dinter J  et al. Different antigen-processing activities in dendritic cells, macrophages, and monocytes lead to uneven production of HIV epitopes and affect CTL recognition. J Immunol 193:4322-34 (2014). Read more (PubMed: 25230751) »

See 1 Publication for this product

客户评价及客户问答

Application
Other
Lane 1 : Fresh Antibody
Lane 2 : Fresh Antibody (Incubation 45 minutes at room temperature)
Lane 3 : Only Second Antibody
Lane 4 : Antibody : Antigen = 1:5 (Incubation 45 minutes at room temperature)
Lane 5 : Antibody : Antigen = 1: 0.05 (Incubation 45 minutes at room temperature)
Lane 6 : Antibody : Antigen = 1: 0.0005 (Incubation 45 minutes at room temperature)
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提交于 Dec 12 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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