概述

  • 产品名称
    人BDNF ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Milk, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 80 pg/ml
  • 范围
    0.066 ng/ml - 16 ng/ml
  • 回收率

    93 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 93.35 83% - 103%
    Serum 94.57 83% - 103%
    Plasma 92.48 82% - 102%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 产品概述

    Abcam’s BDNF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BDNF in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human BDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and BDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BDNF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    BDNF Microplate (12 x 8 wells) 1 unit
    Biotinylated anti-Human BDNF (lyophilized) 2 vials
    Recombinant Human BDNF Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.
  • 细胞定位
    Secreted
  • 别名
    • Abrineurin
    • ANON2
    • Brain derived neurotrophic factor
    • BULN2
    • MGC34632
    • Neurotrophin
    see all
  • 数据库链接

    应用

    Our Abpromise guarantee covers the use of ab99978 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent concentration.

    图片

    • Sandwich ELISA - BDNF Human ELISA Kit (ab99978)
      Sandwich ELISA - BDNF Human ELISA Kit (ab99978)

      Human BDNF measured in cell culture supernatants showing quantity (pg) per mL of tested sample.

    • Sandwich ELISA - BDNF Human ELISA Kit (ab99978)
      Sandwich ELISA - BDNF Human ELISA Kit (ab99978)

      Human BDNF measured in biological fluids showing quantity (pg) per mL of tested sample. Hu plasma diluted 10-270 fold. Hu serum diluted 100-300 fold. Hu milk and saliva diluted 1-3 fold. Mouse and rat samples diluted 1-3 fold.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab99978.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab99978.

    实验方案

    文献

    This product has been referenced in:
    • da Costa RO  et al. The Treadmill Exercise Protects against Dopaminergic Neuron Loss and Brain Oxidative Stress in Parkinsonian Rats. Oxid Med Cell Longev 2017:2138169 (2017). ELISA ; Human . Read more (PubMed: 28713483) »
    • Shi W  et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). Read more (PubMed: 27592818) »

    See all 6 Publications for this product

    发表研究结果有使用 ab99978？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    14 reviews or Q&A

    10
    Votes: Highest First

    Detection of mouse BDNF using the BDNF Human ELISA kit

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    A standard curve using the standards provided, homogenate of mouse cortex, and mouse serum were read in duplicate. Two blanks, where no sample was added, were also read.

    1) 60 mg of cortex was lysed in 800uL of a buffer containing 100mM Tris, 150mM NaCl, 1mM EGTA, 1mM EDTA, 1% Triton, and protease inhibitors. The lysis was done at 4C rotating on a wheel for 2 hours.

    2) lysate centrifuged at 16000xg for 20 minutes collect supernatant

    3) cortex supertnatant was diluted 1:4 and serum was diluted 1:5 in Diluent A for the ELISA

    4) The provided protocol was followed with the standards/samples being incubated with the plate at 4C overnight (step 2 of the Assay Method).

    We were pleased with the results, as we have had trouble finding a reliable ELISA for mouse BDNF.
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Jul 02 2013

    Good Kit

     Good Good 4/5 (Ease of Use)
    Abreviews
    We used the kit to detect BDNF in human neuroblastoma cell line culture supernatants. We performed the assay following the protocol provided in the kit and it worked very good for us.
    Username

    Abcam user community

    Verified customer

    提交于 Jan 24 2018

    Great products and option for mouse BDNF detection

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    This kit initially intended to detect human BDNF, successfully detect also mouse BDNF in brain samples. The kit is easy to use. Good product!
    Username

    Dr. Jianmin Wu

    Verified customer

    提交于 May 26 2016

    I was wondering if it is necessary to filter the media in an attempt to remove cell particulates. We have a filter device that can retain components based on molecular weight. Is it recommended to filter or can I use the conditioned media as it current...

    Read More

    Thank you for contacting us.



    It is not necessary to filter the media, you can use it as is.





    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or i...

    Read More

    I'm using plasma samples, and I was wondering if EDTA is inhibitory with this kit. Also, if samples are hemolyzed will that inhibit the kit in any way?

    During our QC testing, we tested normal serum and plasma samples with a 20 fold dilution to be ˜10ng/ml. As for the storage of the detection antibody, our guarantee for the kit is 6 months if stored at 2° to 8°C. Although not under the guarante...

    Read More

    Quick question about your ELISA for BDNF. Because the proBDNF would also be detected by the mBDNF antibodies, what region does the capture antibody react with? Could one use the capture antibody for both a proBDNF and mBDNF detection antibodies?

    Read More

    The capture and detection antibodies used in this kit both recognize a region within Arg128­-Arg247. Because the propeptide consists of amino acids 19 – 128, this kit would measure both mature and immature BDNF present in your sample.
    Read More

    Is the BDNF measured by the BDNF Human ELISA Kit (ab99978) mature BDNF or pro-BDNF and what is the conversion factor to calculate the concentration in moles?

    The capture and detection antibodies used in this kit both recognize a region within Arg128­-Arg247. Because the propeptide consists of amino acids 19 – 128, this kit is only known to measure the mature form of BDNF. The molecular weight of t...

    Read More

    What MW do I use if I want to calculate molarity? The mature or propetide MW?

    Thank you for contacting us.

    The molecular weight of the standard recombinant BDNF used in this kit (ELH-BDNF-001) is 26,984 Daltons. The sequence of the first five N-terminal amino acids of this BDNF was found to be Met-His-Ser-Asp-Pro. Acces...

    Read More

    I have just downloaded data sheet, but I can't find handling sample urine?

    Can I get prosedure handling for sample urin?

    Thank you

    Thank you for your enquiry and interest in our products.

    Guidelines to prepare urine samples

    1. Collect urine without adding stabilizers.

    2. Centrifuge the samples hard (eg. 10,000 x g for 1 min or 5,000 x g for 2 min). Read More

    .... Unfortunately there was some trouble with the in-house
    delivery of the ordered ELISA-kits, as the package was not directly
    placed in the refrigerator as adviced. I therefore wanted to know if
    there might be a tolerance time in which...

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    Thank you for your email which has been forwarded to me inthe technical support department.

    I am happy to confirm that these kits should not be affected in their performanceif the packagewas not directly placed in therefrigeratorand thatthey ...

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    1-10 of 14 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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