93
%
样品类型 | 平均% | 范围 |
---|---|---|
Cell culture supernatant | 93.35 | 83% - 103% |
Serum | 94.57 | 83% - 103% |
Plasma | 92.48 | 82% - 102% |
Abcam’s BDNF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BDNF in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human BDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and BDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BDNF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
组件 | 1 x 96 tests |
---|---|
200X HRP-Streptavidin Concentrate | 1 x 200µl |
20X Wash Buffer Concentrate | 1 x 25ml |
5X Assay Diluent B | 1 x 15ml |
Assay Diluent A | 1 x 30ml |
BDNF Microplate (12 x 8 wells) | 1 unit |
Biotinylated anti-Human BDNF (lyophilized) | 2 vials |
Recombinant Human BDNF Standard | 2 vials |
Stop Solution | 1 x 8ml |
TMB One-Step Substrate Reagent | 1 x 12ml |
Our Abpromise guarantee covers the use of ab99978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
Human BDNF measured in cell culture supernatants showing quantity (pg) per mL of tested sample.
Human BDNF measured in biological fluids showing quantity (pg) per mL of tested sample. Hu plasma diluted 10-270 fold. Hu serum diluted 100-300 fold. Hu milk and saliva diluted 1-3 fold. Mouse and rat samples diluted 1-3 fold.
Representative Standard Curve using ab99978.
Representative Standard Curve using ab99978.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"