人Bcl-2 ELISA试剂盒(ab119506)

概述

  • 产品名称
    人Bcl-2 ELISA试剂盒
    参阅全部 Bcl-2 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8.6%
    批次间
    样品 n Mean SD CV%
    Overall 12%
  • 样品类型
    Serum, Plasma, Cell culture extracts
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.5 ng/ml
  • 范围
    0.5 ng/ml - 32 ng/ml
  • 复检

    71 %

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Human Bcl-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab119506) is designed for accurate quantitative measurement of Human Bcl-2 concentrations in cell lysates, serum and plasma.


    Bcl-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Bcl-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bcl-2 captured on the plate.


    Get results in 90 minutes with Human Bcl-2 ELISA Kit (ab202411) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-human Bcl-2 monoclonal antibody 1 x 70µl
    Human Bcl-2 Standard lyophilized (64 ng/mL upon reconstitution) 2 vials
    Lysis Buffer (10x) 1 x 15ml
    Microplate coated with monoclonal antibody to Bcl-2 (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 功能
    Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • 组织特异性
    Expressed in a variety of tissues.
  • 疾病相关
    A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • 序列相似性
    Belongs to the Bcl-2 family.
  • 结构域
    BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
    The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
    The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
  • 翻译后修饰
    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
  • 细胞定位
    Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 别名
    • Apoptosis regulator Bcl 2
    • Apoptosis regulator Bcl-2
    • Apoptosis regulator Bcl2
    • AW986256
    • B cell CLL/lymphoma 2
    • B cell leukemia/lymphoma 2
    • Bcl-2
    • Bcl2
    • BCL2_HUMAN
    • C430015F12Rik
    • D630044D05Rik
    • D830018M01Rik
    • Leukemia/lymphoma, B-cell, 2
    • Oncogene B-cell leukemia 2
    • PPP1R50
    • Protein phosphatase 1, regulatory subunit 50
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab119506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119506.

实验方案

文献

ab119506 has not yet been referenced specifically in any publications.

客户评价及客户问答

While we do not have binding information available, AB119506, BML2 assay, detected Bcl-2 in pathological sera from patients with melanoma. The originating lab also performed protein spike recovery assays.

Hello!

This kit does recognizes both the alpha and beta isoform of the Bcl-2 protein.

Please let me know if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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