人BAFF ELISA试剂盒(TNFSF13B) (ab188391)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12.7 pg/ml
- Range: 0.08 ng/ml - 5 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人BAFF ELISA试剂盒(TNFSF13B)
参阅全部 BAFF 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 8 8.3% 批次间 样品 n Mean SD CV% Overall 3 8.8% -
样品类型
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
12.7 pg/ml -
范围
0.08 ng/ml - 5 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 111 105% - 115% Cell culture media 108 106% - 110% Hep Plasma 104 101% - 108% EDTA Plasma 105 98% - 115% Cit plasma 106 100% - 111% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human BAFF ELISA Kit (TNFSF13B) (ab188391) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BAFF (TNFSF13B) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BAFF (TNFSF13B) with 12.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
B-cell activating factor (BAFF) protein that is encoded by the TNFSF13B gene. BAFF protein is expressed in B-cell lineage cells which functions as a cytokine and is also a member of the tumor necrosis factor (TNF) family of ligands. The BAFF cytokine acts as a potent B-cell activator promoting the proliferation and differentiation of B-cells. This protein is expressed as a membrane-bound type II transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. The membrane bound form can be cleaved from the membrane, generating a soluble protein fragment. BAFF steady-state concentrations depend on B-cells and also on the expression of BAFF-binding receptors.
Inadequate levels of BAFF will fail to activate B-cells to produce enough immunoglobulin and will lead to immunodeficiency. Excessive level of BAFF causes abnormally high antibody production, results in systemic lupus erythmatosis, rheumatoid arthritis, and many other autoimmune diseases.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human BAFF Capture Antibody 1 x 600µl 10X Human BAFF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human BAFF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
组织特异性
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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别名
- ApoL related ligand TALL 1
- B cell Activating Factor
- B lymphocyte stimulator
see all -
数据库链接
- Entrez Gene: 10673 Human
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- Unigene: 525157 Human
- Unigene: 596110 Human
相关产品
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ELISA kits
图片
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Quantification of BAFF interpolated from standard curve and multiplied by dilution factor.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab188391 被引用在 3 文献中.
- Hinterleitner M et al. Early Reconstitution of Antibody Secreting Cells after Allogeneic Stem Cell Transplantation. J Clin Med 11:N/A (2022). PubMed: 35012014
- Cao Y et al. BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF-?B signaling pathway in glomerular mesangial cells. Mol Med Rep 21:795-805 (2020). PubMed: 31974601
- Alkhairy OK et al. RAC2 loss-of-function mutation in 2 siblings with characteristics of common variable immunodeficiency. J Allergy Clin Immunol N/A:N/A (2014). ELISA ; Human . PubMed: 25512081