人BAFF ELISA试剂盒(TNFSF13B) (ab119579)

概述

  • 产品名称人BAFF ELISA试剂盒(TNFSF13B)
    参阅全部 BAFF 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    62.5 pg/ml - 4000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human BAFF (TNFSF13B) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BAFF (TNFSF13B) in cell culture supernatants, serum and plasma (heparin, EDTA).

    A BAFF (TNFSF13B) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BAFF (TNFSF13B) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BAFF (TNFSF13B) amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
    Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.
  • 组织特异性Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines.
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
    Isoform 2 is not efficiently shed from the membrane unlike isoform 1.
    N-glycosylated.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 别名
    • B lymphocyte stimulator
    • B-cell-activating factor
    • BAFF
    • BLyS
    • CD257
    • Dendritic cell-derived TNF-like molecule
    • soluble form
    • TALL-1
    • TN13B_HUMAN
    • TNF- and APOL-related leukocyte expressed ligand 1
    • TNFSF13B
    • Tumor necrosis factor ligand superfamily member 13b
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human BAFF ELISA Kit (TNFSF13B) 图像

  • Representative Standard Curve using ab119579.

实验方案

Human BAFF ELISA Kit (TNFSF13B) (ab119579)参考文献

ab119579 has not yet been referenced specifically in any publications.

Product Wall



1.) Yes, a washing step is needed. This washing step is carried out when following the preparation of the plate under point 7 A.

This plate preparation should be done immediately before starting the assay procedure.

2.) No...

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The strips are divisible and precoated with antibodies, so you can use the wells directly.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"