人BAFF ELISA试剂盒(TNFSF13B) (ab119579)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人BAFF ELISA试剂盒(TNFSF13B)
参阅全部 BAFF 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 127pg/ml 5.46 = 4.3% 2 16 522pg/ml 38.62 = 7.4% 3 16 1229pg/ml 79.88 = 6.5% 批次间 样品 n Mean SD CV% 1 24 124pg/ml 5.7 = 4.6% 2 24 548pg/ml 47.67 = 8.7% 3 24 1268pg/ml 103.97 = 8.2% -
样品类型
Cell culture supernatant, Serum, Plasma, Cell Lysate -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human BAFF (TNFSF13B) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BAFF (TNFSF13B) in cell culture supernatants, serum and plasma (heparin, EDTA).
A BAFF (TNFSF13B) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BAFF (TNFSF13B) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BAFF (TNFSF13B) amount of sample captured in plate.
Get higher sensitivity in only 90 minutes with Human BAFF ELISA Kit (TNFSF13B) (ab188391) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human BAFF Antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-Human BAFF antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Human BAFF standard 2 x 10ng 2 x 10ng Plate Seal 4 units 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究领域
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功能
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
组织特异性
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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别名
- ApoL related ligand TALL 1
- B cell Activating Factor
- B lymphocyte stimulator
see all -
数据库链接
- Entrez Gene: 10673 Human
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- Unigene: 525157 Human
- Unigene: 596110 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab119579 被引用在 2 文献中.
- Patgaonkar M et al. Vivax infection alters peripheral B-cell profile and induces persistent serum IgM. Parasite Immunol 40:e12580 (2018). PubMed: 30102786
- Mameli G et al. Serum BAFF levels, Methypredsinolone therapy, Epstein-Barr Virus and Mycobacterium avium subsp. paratuberculosis infection in Multiple Sclerosis patients. Sci Rep 6:29268 (2016). PubMed: 27383531