The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
纯度70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
APP beta secretase
Aspartyl protease 2
Beta secretase 1
Beta site amyloid beta A4 precursor protein cleaving enzyme
Beta site amyloid precursor protein cleaving enzyme
Beta site amyloid precursor protein cleaving enzyme 1
Beta site APP cleaving enzyme
Beta site APP cleaving enzyme 1
Beta-site amyloid precursor protein cleaving enzyme 1
Beta-site APP cleaving enzyme 1
Membrane associated aspartic protease 2
Membrane-associated aspartic protease 2
Transmembrane aspartic proteinase Asp2
功能Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
组织特异性Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
序列相似性Belongs to the peptidase A1 family.
结构域The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
细胞定位Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.