概述

  • 产品名称
    人Axl ELISA试剂盒
    参阅全部 Axl 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    2.74 pg/ml - 2000 pg/ml
  • 复检

    < 94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 92.35 82% - 102%
    Serum 91.77 82% - 102%
    Plasma 93.49 83% - 103%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Axl Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Axl in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Axl coated on a 96-well plate. Standards and samples are pipetted into the wells and Axl present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Axl antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Axl bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab99976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab99976.

  • Representative Standard Curve using ab99976.

实验方案

文献

This product has been referenced in:
  • Xu J  et al. Identification of the soluble form of tyrosine kinase receptor Axl as a potential biomarker for intracranial aneurysm rupture. BMC Neurol 15:23 (2015). Human . Read more (PubMed: 25885003) »

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting us. I have had the opportunity to discuss your question with the lab. They have informed me that the antibody pair in the ab99976 recognizes a region on the extracellular domain. Therefore the soluble form of this receptor i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册