概述

  • 产品名称人ARP4 ELISA试剂盒(Angiopoietin-Like 4)
  • 检测方法Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    27.4 pg/ml - 20000 pg/ml
  • 复检

    100.4 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 117.8 67% - 137%
    Serum 95.38 85% - 104%
    Plasma 88.04 74% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s ARP4 (Angiopoietin-like 4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ARP4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human ARP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and ARP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ARP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ARP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性ARP4 is a fasting-induced adipose factor. It was identified as an adipocytokine up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. At the protein level, in human and mouse blood plasma, ARP4 was found to be present both as a native protein and in a truncated form. Differentiation of mouse 3T3-L1 adipocytes was associated with the production of truncated ARP4, whereas in human white adipose tissue and SGBS adipocytes, only the native ARP4 could be detected. Interestingly, the truncated ARP4 was produced by human liver. Experimental data suggest that ARP4 is mainly presented in human blood plasma in a truncated form (ARP4-S2), whose level is increased by fenofibrate treatment. Levels of both truncated and native ARP4 showed marked inter individual variation but were not associated with body mass index and were not influenced by prolonged semistarvation.
  • 别名
    • 425O18 1
    • Angiopoietin like 4
    • Angiopoietin related protein 4
    • ANGPT L2
    • ANGPT L4
    • ANGPTL2
    • ANGPTL4
    • Fasting induced adipose factor
    • FIAF
    • Fibrinogen/angiopoietin related protein
    • Hepatic fibrinogen/angiopoietin related protein
    • HFARP
    • Major histocompatibility complex region NG27
    • NL2
    • Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein
    • PGAR
    • pp1158
    • PPARG angiopoietin related protein
    • PSEC0166
    • Secreted protein Bk89
    • Weakly similar to angiopoietin 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human ARP4 ELISA Kit (Angiopoietin-Like 4) 图像

  • Representative standard curve using ab99974.

  • Representative standard curve using ab99974.

实验方案

Human ARP4 ELISA Kit (Angiopoietin-Like 4) (ab99974)参考文献

This product has been referenced in:
  • Frenzel E  et al. a1-Antitrypsin Combines with Plasma Fatty Acids and Induces Angiopoietin-like Protein 4 Expression. J Immunol 195:3605-16 (2015). Read more (PubMed: 26363050) »
  • Zhao X  et al. Clostridium butyricum reduce lipogenesis through bacterial wall components and butyrate. Appl Microbiol Biotechnol 98:7549-57 (2014). Read more (PubMed: 24878750) »

See all 3 Publications for this product

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