概述

  • 产品名称
    人APRIL ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8.1%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.4 ng/ml
  • 范围
    0.78 ng/ml - 50 ng/ml
  • 复检

    76 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 76 % - %

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s APRIL Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human APRIL concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).

    APRIL specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an APRIL biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of APRIL captured on the plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-human APRIL polyclonal antibody 1 x 70µl
    Human APRIL Standard 2 vials
    Microplate coated with polyclonal antibody to Human APRIL (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 功能
    Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
  • 组织特异性
    Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
  • 序列相似性
    Belongs to the tumor necrosis factor family.
  • 翻译后修饰
    The precursor is cleaved by furin.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • A proliferation-inducing ligand
    • APRIL
    • CD256
    • TALL-2
    • TALL2
    • TNF- and APOL-related leukocyte expressed ligand 2
    • TNF-related death ligand 1
    • TNF13_HUMAN
    • TNFSF13
    • TRDL-1
    • Tumor necrosis factor (ligand) superfamily member 13
    • Tumor necrosis factor ligand superfamily member 13
    • Tumor necrosis factor like protein ZTNF2
    • Tumor necrosis factor related death ligand 1
    • ZTNF2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119505.

实验方案

文献

ab119505 has not yet been referenced specifically in any publications.

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