概述

  • 产品名称人APRIL ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8.1%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.4 ng/ml
  • 范围
    0.78 ng/ml - 50 ng/ml
  • 复检

    76 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 76 % - %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s APRIL Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human APRIL concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).

    APRIL specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an APRIL biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of APRIL captured on the plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
  • 组织特异性Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The precursor is cleaved by furin.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • A proliferation-inducing ligand
    • APRIL
    • CD256
    • TALL-2
    • TALL2
    • TNF- and APOL-related leukocyte expressed ligand 2
    • TNF-related death ligand 1
    • TNF13_HUMAN
    • TNFSF13
    • TRDL-1
    • Tumor necrosis factor (ligand) superfamily member 13
    • Tumor necrosis factor ligand superfamily member 13
    • Tumor necrosis factor like protein ZTNF2
    • Tumor necrosis factor related death ligand 1
    • ZTNF2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human APRIL ELISA Kit 图像

  • Representative Standard Curve using ab119505.

实验方案

Human APRIL ELISA Kit (ab119505)参考文献

ab119505 has not yet been referenced specifically in any publications.

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