人Apoptosis抗体Array -膜(43 Targets) (ab134001)

概述

  • 产品名称
    人Apoptosis抗体阵列-膜(43 Targets)
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型
    Semi-quantitative
  • 种属反应性
    与反应: Human
  • 产品概述

    ab134001 is for simultaneous detection of 43 Human Apoptotic markers. Suitable for cell and tissue lysates.


    Targets: Bad, Bax, Bcl-2, Bcl-w, BID, BIM, Caspase3, caspase8, CD40, CD40L, cIAP-2, cytoC, DR6, Fas, FasL, HSP27, HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1sR, livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-alpha, TNF-beta, TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4, XIAP


    Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • 经测试应用
    适用于: Multiplex Protein Detectionmore details

性能

应用

Our Abpromise guarantee covers the use of ab134001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Multiplex Protein Detection Use at an assay dependent concentration.

图片

  • Apoptotic protein profiling in Staurosporine-treated and untreated Jurkat cell lines

    107 Jurkat cells were untreated or treated with staurosporine (0.5 µM) for 3 hours. Equal total lysate protein was loaded on each Abcam Apoptosis Antibody Array membrane. Antibody spots exhibiting signal differences are indicated in red boxes.

实验方案

文献

ab134001 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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