人Apoptosis抗体Array -膜(43 Targets) (ab134001)

概述

  • 产品名称
    人Apoptosis抗体阵列-膜(43 Targets)
  • 样品类型
    Tissue Extracts, Cell Lysate
  • 检测类型
    Semi-quantitative
  • 种属反应性
    与反应: Human
  • 产品概述

    ab134001 is for simultaneous detection of 43 Human Apoptotic markers. Suitable for cell and tissue lysates.


    Targets: Bad, Bax, Bcl-2, Bcl-w, BID, BIM, Caspase3, caspase8, CD40, CD40L, cIAP-2, cytoC, DR6, Fas, FasL, HSP27, HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1sR, livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-alpha, TNF-beta, TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4, XIAP


    Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • 经测试应用
    适用于: Multiplex Protein Detectionmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab134001 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Multiplex Protein Detection Use at an assay dependent concentration.

    Human Apoptosis Antibody Array - Membrane (43 Targets) 图像

    • Apoptotic protein profiling in Staurosporine-treated and untreated Jurkat cell lines

      107 Jurkat cells were untreated or treated with staurosporine (0.5 µM) for 3 hours. Equal total lysate protein was loaded on each Abcam Apoptosis Antibody Array membrane. Antibody spots exhibiting signal differences are indicated in red boxes.

    实验方案

    Human Apoptosis Antibody Array - Membrane (43 Targets) (ab134001)参考文献

    ab134001 has not yet been referenced specifically in any publications.

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