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ab134001 is for simultaneous detection of 43 Human Apoptotic markers. Suitable for cell and tissue lysates.
Targets: Bad, Bax, Bcl-2, Bcl-w, BID, BIM, Caspase3, caspase8, CD40, CD40L, cIAP-2, cytoC, DR6, Fas, FasL, HSP27, HSP60, HSP70, HTRA, IGF-I, IGF-II, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1sR, livin, p21, p27, p53, SMAC, Survivin, sTNF-R1, sTNF-R2, TNF-alpha, TNF-beta, TRAILR-1, TRAILR-2, TRAILR-3, TRAILR-4, XIAP
Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
|组件||1 x 4 Membranes||1 x 8 Membranes||1 x 2 Membranes|
|1,000X HRP-Streptavidin Buffer||1 x 50µl||1 x 50µl||1 x 50µl|
|Blocking Buffer||1 x 25ml||1 x 50ml||1 x 25ml|
|20X Wash Buffer I||1 x 10ml||1 x 20ml||1 x 10ml|
|20X Wash Buffer II||1 x 10ml||1 x 20ml||1 x 10ml|
|2X Cell Lysis Buffer||1 x 10ml||1 x 20ml||1 x 10ml|
|8-Well Incubation Tray (with Lid)||1 unit||1 unit||1 unit|
|Apoptosis Antibody Array Membranes||4 units||8 units||2 units|
|Biotin-Conjugated Anti-Cytokines||2 vials||4 vials||1 vial|
|Detection Buffer C||1 x 1.5ml||1 x 2.5ml||1 x 1.5ml|
|Detection Buffer D||1 x 1.5ml||1 x 2.5ml||1 x 1.5ml|
|Protease Inhibitor Cocktail||1 vial||2 vials||1 vial|
Our Abpromise guarantee covers the use of ab134001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
Apoptotic protein profiling in Staurosporine-treated and untreated Jurkat cell lines
107 Jurkat cells were untreated or treated with staurosporine (0.5 µM) for 3 hours. Equal total lysate protein was loaded on each Abcam Apoptosis Antibody Array membrane. Antibody spots exhibiting signal differences are indicated in red boxes.
ab134001 has not yet been referenced specifically in any publications.