人Apolipoprotein H ELISA试剂盒(APOH) (ab108814)

概述

  • 产品名称
    人Apolipoprotein H ELISA试剂盒(APOH)
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.2%
    批次间
    样品 n Mean SD CV%
    Overall 10%
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Cell Lysate
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 0.45 ng/ml
  • 范围
    0.625 ng/ml - 40 ng/ml
  • 复检

    98 %

  • 检测时间
    4h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Apolipoprotein H (APOH) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein H concentrations in Human plasma, serum, saliva, urine, milk and cell culture samples.

    An Apolipoprotein H specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein H specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein H captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Apolipoprotein H measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108814

实验方案

文献

ab108814 has not yet been referenced specifically in any publications.

客户评价及客户问答



Our guarantee period is 12 month from the date of purchase and therefore I suggest to also take this date as expiry date for un-opened kits that are stored as described in the protocol booklet.

When reagents are opened they also can...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册