人Apolipoprotein B ELISA试剂盒(APOB) (ab108807)

概述

  • 产品名称人Apolipoprotein B ELISA试剂盒(APOB)
    参阅全部 Apolipoprotein B 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5%
    批次间
    样品 n Mean SD CV%
    Overall 9.4%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Cell culture extracts, Cell Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.008 µg/ml
  • 范围
    0.008 µg/ml - 0.25 µg/ml
  • 复检

    98 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Apolipoprotein B (APOB) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein B concentrations in Human plasma, serum, CSF and cell culture samples.


    An Apolipoprotein B specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein B specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein B captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Apolipoprotein B ELISA Kit (ab190806) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • 疾病相关Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • 序列相似性Contains 1 vitellogenin domain.
  • 翻译后修饰Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • 细胞定位Cytoplasm. Secreted.
  • Information by UniProt
  • 别名
    • Apo B-100
    • Apo B-48
    • APOB
    • ApoB 100
    • ApoB 48
    • APOB_HUMAN
    • Apolipoprotein B
    • Apolipoprotein B 100
    • Apolipoprotein B-48
    • Apolipoprotein B48
    • FLDB
    • LDLCQ4
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Apolipoprotein B ELISA Kit (APOB) 图像

  • Apolipoprotein B measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108807

实验方案

Human Apolipoprotein B ELISA Kit (APOB) (ab108807)参考文献

ab108807 has not yet been referenced specifically in any publications.

Product Wall

Merci pour votre émail.

Nous n'avons pas besoin de plus de formalités. Je vais mettre en l'envoi du nouveau kit.

Nous sommes désolés que l'ancien kit que vous avez reçu ne fonctionne pas comme attendu. Voici le numéro de commande ...

Read More

Only the assays for APOB (ab108807) and APOE (ab108813) have been tested with CSF. They are suitable for this kind of sample. The APOA1 assays have not been tested with CSF yet, but there are plans to test ab108803. Kit ab108804, the competitive ELISA ...

Read More


Me temo que el kit ab108807 no puede distinguir entre las isoformas Apo B48 y Apo B100.

The plate is Corning Costar® 2592. It should fit into most plate washers. The wash buffer contains 0.1% Tween -20.



Q1: Will this kit detect mouse ApoB?
A1: No.

Q2: Is it known whether this kit will detect ApoB48 or ApoB100? Or both?
A2: It has not been determined if this kit will specifically detect ApoB48 or ApoB100.

Thank you for your inquiry and I apologize for the delay in this reply.

The lyophilized powder standard is supplied at 0.75 ug. Reconstitute the 0.75 ug of Apo B Standard with 3 ml of EIA Diluent to generate a solution of 0.25 ug/ml. Any left...

Read More

Hello. The cross reactivity was deternmined by purified protein and plasma samples combination. It is not by single determinants. Please let me know if you have any further questions.



I believe this kit includes 0.75ug of standard. Based on the protocol, this provides you 3 mls of 0.25ug/ml of the highest concentration of standard. This starting concentration is subsequently diluted in 2x serial dilutions to produce the cu...

Read More

Thank you for contacting us. There is no cross-reactivity with mouse for ab108807. Please let me know if I can be of further assistance.


Here is the shelf life information you requested:
ab108804 - 6 months if kit is unused and reagents stored at 4 deg C or -20 deg C as appropriate; 1 month after opening and limiting reagent is microtiter plate storage
ab108807 - 6 months...

Read More

1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"