人Apolipoprotein AII ELISA试剂盒(APOA2) (ab108805)

概述

  • 产品名称人Apolipoprotein AII ELISA试剂盒(APOA2)
    参阅全部 Apolipoprotein AII 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.7%
    批次间
    样品 n Mean SD CV%
    Overall 7.2%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Cell culture extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.004 µg/ml
  • 范围
    0.004 µg/ml - 4 µg/ml
  • 复检

    97 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Apolipoprotein AII (APOA2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AII concentrations in cell culture samples, serum and plasma, urine and milk.


    An Apolipoprotein AII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AII captured in plate.


    For higher reproducibility in only 90 minutes, we recommend our SimpleStep ELISA® range made with recombinant monoclonal antibodies. Try Human Apolipoprotein AII ELISA Kit (ab184859).

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
  • 细胞定位Secreted.
  • 别名
    • Apo-AII
    • Apolipoprotein A-II
    • Apolipoprotein A2
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab108805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Apolipoprotein AII ELISA Kit (APOA2) 图像

  • Representative Standard Curve using ab108805

实验方案

Human Apolipoprotein AII ELISA Kit (APOA2) (ab108805)参考文献

ab108805 has not yet been referenced specifically in any publications.

Product Wall

Abreviews

Sample: Human
Amount of Sample: 0-20 µg
Type of Kit Used: ELISA
Positive control: APOB, APOAII, HDL, LDL
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Kit detects APOB
Abcam response: We thank the customer for this feedback and we are currently working to resolve this issue.
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Verified customer

提交于 May 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"