人Apolipoprotein AI ELISA试剂盒(APOA1) (ab108803)

概述

  • 产品名称人Apolipoprotein AI ELISA试剂盒(APOA1)
    参阅全部 Apolipoprotein A I 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.8%
    批次间
    样品 n Mean SD CV%
    Overall 9.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Cell culture extracts, Cerebral Spinal Fluid
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 3 ng/ml
  • 范围
    3.13 ng/ml - 200 ng/ml
  • 复检

    98 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Apolipoprotein AI (APOA1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AI concentrations in urine, saliva, milk, CSF and cell culture samples.


    An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AI captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Apolipoprotein AI ELISA Kit (ab189576) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 组织特异性Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
  • 疾病相关High density lipoprotein deficiency 2
    High density lipoprotein deficiency 1
    APOA1 mutations may be involved in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III (PubMed:3142462 and PubMed:2123470). The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis.
    Amyloidosis 8
  • 序列相似性Belongs to the apolipoprotein A1/A4/E family.
  • 翻译后修饰Palmitoylated.
    Met-110 and Met-136 are oxidized to methionine sulfoxides.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Apo-AI
    • ApoA-I
    • APOA1
    • APOA1_HUMAN
    • Apolipoprotein A-I
    • Apolipoprotein A-I(1-242)
    • Apolipoprotein A1
    • Truncated apolipoprotein A-I
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Apolipoprotein AI ELISA Kit (APOA1) 图像

  • APOA1 measured in various samples showing quantity (microgram) per mL of tested sample

  • Representative Standard Curve using ab108803

实验方案

Human Apolipoprotein AI ELISA Kit (APOA1) (ab108803)参考文献

ab108803 has not yet been referenced specifically in any publications.

Product Wall


The amount of standard for lot GR132237-1 = 3.2 ug (micrograms).

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