人Antithrombin III ELISA试剂盒(ab108801)

概述

  • 产品名称
    人Antithrombin III ELISA试剂盒
    参阅全部 Antithrombin III 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.8%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture media, Cerebral Spinal Fluid
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 3 ng/ml
  • 范围
    3.13 ng/ml - 200 ng/ml
  • 复检

    98.5 %

  • 检测时间
    4h 0m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Antithrombin III Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III in urine, plasma, milk, saliva, cerebrospinal fluid, serum, and cell culture supernatants.


    An antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of antithrombin III captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 功能
    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • 组织特异性
    Found in plasma.
  • 疾病相关
    Antithrombin III deficiency
  • 序列相似性
    Belongs to the serpin family.
  • 翻译后修饰
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位
    Secreted > extracellular space.
  • Information by UniProt
  • 别名
    • ANT3_HUMAN
    • Antithrombin-III
    • ATIII
    • Serpin C1
    • SERPINC1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Antithrombin III ELISA Kit 图像

  • Linearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution  (duplicates +/- SD)

  • Linearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution (duplicates +/- SD), showing ng x mL-1 on y-axis.

  • Linearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution  (duplicates +/- SD)

  • Antithrombin III in cell culture supernatants (duplicates +/- SD), tested in the dilution range of 1:1-1:100.

  • Antithrombin III in human biological fluids (duplicates +/- SD). The samples were differently diluted  (serum and Li-heparine plasma: 1e4-1e5; CSF: 1e2-1e3; milk: 10-100; saliva: 5-100; urine: neat)

  • Representative standard curve using ab108801.

实验方案

Human Antithrombin III ELISA Kit (ab108801)参考文献

ab108801 has not yet been referenced specifically in any publications.

Product Wall

Please find below the information on how to prepare the standard for lots GR116626-1, Lot GR115289-1 and Lot GR106377-1 of the Antithrombin III Human ELISA Kit ab108801:


· MIX Diluent Concentrate (10x): If crystals have formed in t...

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