人anti-Annexin V ELISA试剂盒(ab119504)

概述

  • 产品名称
    人anti-Annexin V ELISA试剂盒
    参阅全部 Annexin V 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 2%
    批次间
    样品 n Mean SD CV%
    Overall 3.8%
  • 样品类型
    Cell culture supernatant
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    1.2 ng/ml
  • 范围
    6.3 ng/ml - 400 ng/ml
  • 复检

    86 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 86 74% - 100%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s anti-Annexin V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human anti-Annexin V antibody concentrations in cell culture supernatant.

    A 96-well plate has been precoated with Annexin V to which cognate antibodies bind. Standards or test samples are added to the wells and incubated. A horseradish peroxidase (HRP) labeled Annexin V conjugate is added to the wells, which is bound by the immobilized Annexin V-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of anti-Annexin V antibodies sample captured in the plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119504.

实验方案

文献

ab119504 has not yet been referenced specifically in any publications.

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