人anti-Annexin V ELISA试剂盒(ab119504)

概述

  • 产品名称人anti-Annexin V ELISA试剂盒
    参阅全部 Annexin V 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 2%
    批次间
    样品 n Mean SD CV%
    Overall 3.8%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    1.2 ng/ml
  • 范围
    6.3 ng/ml - 400 ng/ml
  • 复检

    86 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 86 74% - 100%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s anti-Annexin V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human anti-Annexin V antibody concentrations in cell culture supernatant.

    A 96-well plate has been precoated with Annexin V to which cognate antibodies bind. Standards or test samples are added to the wells and incubated. A horseradish peroxidase (HRP) labeled Annexin V conjugate is added to the wells, which is bound by the immobilized Annexin V-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of anti-Annexin V antibodies sample captured in the plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 疾病相关Pregnancy loss, recurrent, 3
  • 序列相似性Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • 别名
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human anti-Annexin V ELISA Kit 图像

  • Representative Standard Curve using ab119504.

实验方案

Human anti-Annexin V ELISA Kit (ab119504)参考文献

ab119504 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119504.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"