人Annexin V ELISA试剂盒(ab119503)

概述

  • 产品名称人Annexin V ELISA试剂盒
    参阅全部 Annexin V 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall 11%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.33 ng/ml
  • 范围
    0.78 ng/ml - 50 ng/ml
  • 复检

    85 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Annexin V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Annexin V concentrations in cell culture supernatant and serum.

    Annexin V specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Annexin V detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Annexin V sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • 疾病相关Pregnancy loss, recurrent, 3
  • 序列相似性Belongs to the annexin family.
    Contains 4 annexin repeats.
  • 结构域The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    A pair of annexin repeats may form one binding site for calcium and phospholipid.
  • 翻译后修饰S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Information by UniProt
  • 别名
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP-I
    • Endonexin II
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PLACENTAL PROTEIN 4
    • Pp4
    • Thromboplastin inhibitor
    • VAC-alpha
    • Vascular anticoagulant-alpha
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Annexin V ELISA Kit 图像

  • Representative Standard Curve using ab119503.

实验方案

Human Annexin V ELISA Kit (ab119503)参考文献

ab119503 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your inquiry.

Once the standard is reconstituted, we recommend using them immediately and then discarding the remaining portion.
We have not performed stability studies regarding the long -term storage of reconsti...

Read More

Once the standard is reconstituted, we recommend using them immediately and then discarding the remaining portion.


We have not performed stability studies regarding the long -term storage of reconstituted standard protein so unfortunate...

Read More

Thank you for contacting us. The reconstitution instructions for the standard are lot specific and will be written on the vial. If for any reason it is not, please let us know your lot number and we will be happy to find that information for you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"