人Angiostatin ELISA试剂盒(ab99973)

概述

  • 产品名称人Angiostatin ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 20 ng/ml
  • 范围
    31.25 ng/ml - 2000 ng/ml
  • 复检

    < 95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.43 83% - 104%
    Serum 92.45 81% - 101%
    Plasma 93.31 82% - 102%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Angiostatin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiostatin in serum, plasma (heparin) and cell culture supernatants.

    This assay employs an antibody specific for Human Angiostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Angiostatin is an internal proteolytic fragment of plasminogen, containing at least three Kringle domains. The Kringle domains have specific anti-migratory and anti-proliferative activities. Angiostatin, an endogenous angiogenesis inhibitor, inhibits endothelial cell proliferation in vitro and angiogenesis in vivo. It is a potent and specific natural inhibitor of angiogenisis and metastatic tumor growth.
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Angiostatin ELISA Kit 图像

  • Titration of NIBSC Plasmin (97/536), with background signal subtracted (duplicates; +/- SD).

  • Angiostatin measured in human undiluted biological fluids (duplicates +/- SD). Signals from mouse and rat (pooled) plasma and sera were below level of detection.

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab99973.

实验方案

Human Angiostatin ELISA Kit (ab99973)参考文献

ab99973 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your call last week and for your patience while I have been in touch with the lab regarding your question.
My contact at the lab has said that the plates are optimized with the provided wash buffer, but it is a PBS-based bu...

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The lab confirmed that the assay will detect plasminogen, in addition to angiostatin. I am going to ask that we change the name of the assay to anti - Angiostatin/Plasminogen, or at least add a note indicating reactivity with plasminogen. Thank you for...

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The capture antibody used in this ELISA (ELH-Angiostatin-001) is a mouse monoclonal that recognizes the amino acid sequence of 98 – 358 of human Angiostatin Kringle 1 – 3. The detection antibody is a goat polyclo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"