概述

  • 产品名称人Angiopoietin 2 ELISA试剂盒(ANG2)
    参阅全部 Angiopoietin 2 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    4.12 pg/ml - 3000 pg/ml
  • 复检

    88 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 82.1 76% - 92%
    Serum 85.3 82% - 109%
    Plasma 96.9 95% - 116%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Angiopoietin 2 (ANG2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 2 in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for Human Angiopoietin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • 序列相似性Contains 1 fibrinogen C-terminal domain.
  • 结构域The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • AGPT2
    • ANG 2
    • ANG-2
    • ANG2
    • Angiopoietin 2a
    • Angiopoietin 2B
    • Angiopoietin-2
    • Angiopoietin2
    • ANGP2_HUMAN
    • ANGPT 2
    • Angpt2
    • Tie2 ligand
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Angiopoietin 2 ELISA Kit (ANG2) 图像

  • Representative Standard Curve using ab99971.

实验方案

Human Angiopoietin 2 ELISA Kit (ANG2) (ab99971)参考文献

This product has been referenced in:
  • Genre F  et al. Antitumour necrosis factor-a therapy modulates angiopoietin-2 serum levels in non-diabetic ankylosing spondylitis patients. Ann Rheum Dis 72:1265-7 (2013). Read more (PubMed: 23482472) »

See 1 Publication for this product

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