人Angiopoietin 1 ELISA试剂盒(ANG1) (ab99972)

概述

  • 产品名称人Angiopoietin 1 ELISA试剂盒(ANG1)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 30 pg/ml
  • 范围
    74 pg/ml - 18000 pg/ml
  • 复检

    < 95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.25 83% - 105%
    Serum 94.47 81% - 103%
    Plasma 93.93 80% - 102%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Angiopoietin 1 (ANG1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Angiopoietin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Angiopoietin 1 is one of the Angiopoietin (Ang) family of growth factors. It binds and activates the TIE2 receptor tyrosine kinase by inducing its tyrosine phosphorylation. It is implicated in endothelial developmental processes later and distinct from that of VEGF. The structure of angiopoietin 1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Angiopoietin 1 appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It also mediates blood vessel maturation/stability and it may play an important role in the heart early development. Angiopoietin 1 may have a potential therapeutic utility since it can be used for specifically targeting tumor vasculature or for promoting angiogenic processes in certain organs such as an ischemic heart.
  • 别名
    • 1110046O21Rik
    • AGP 1
    • AGP1
    • AGPT
    • ANG 1
    • ANG1
    • Angiopoietin1
    • ANGPT 1
    • ANGPT1
    • KIAA0003
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Angiopoietin 1 ELISA Kit (ANG1) 图像

  • TYPICAL STANDARD CURVE - Assay DIluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
  • TYPICAL STANDARD CURVE - Assay Diluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

实验方案

Human Angiopoietin 1 ELISA Kit (ANG1) (ab99972)参考文献

This product has been referenced in:
  • Ollivier V  et al. Collagen can selectively trigger a platelet secretory phenotype via glycoprotein VI. PLoS One 9:e104712 (2014). Read more (PubMed: 25116206) »

See 1 Publication for this product

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